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名稱	重組蛋白Human sRANK Ligand
型號
更新時間	2023-09-25
特點	RANKL和等級是TNF超科的成員發(fā)揮重要作用的配體和受體的規(guī)定特定的免疫和骨代謝。RANKL的表達(dá)在多種細(xì)胞包括成骨細(xì)胞、成纖維細(xì)胞、激活t細(xì)胞和骨髓基質(zhì)細(xì)胞,可溶性sRANKL抑制osteoclastogenesis打斷基質(zhì)細(xì)胞之間的信號和監(jiān)測祖細(xì)胞,從而導(dǎo)致骨骼和軟骨的過度積累。重組人類sRANKL 20.0 kDa多肽包括TNF同源的RANKL地區(qū)(176氨基酸殘基)。}

詳細(xì)內(nèi)容

一.產(chǎn)品名稱：Recombinant Human sRANK Ligand

二.Catalog Number:310-01

三.Synonyms:

soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE (TNF-related activation-induced cytokine), OPGL, ODF (Osteoclast differentiation factor)

四.Description:

RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant human sRANKL is a 20.0 kDa polypeptide comprising the TNF homologous region of RANKL (176 amino acid residues).

五.AA Sequence:

MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID

六.Source:E.coli

七.Purity:Greater than 98% by SDS-PAGE gel and HPLC analyses.

八.Biological Activity:

Determined by its ability to induce NFkappaB in RAW264.7 cells in the absence of any cross-linking. The expected ED₅₀ for this effect is 10.0-25.0 ng/ml.

九.Cross Reactivity:Mouse, Rat, Human

十.www.peprotech.com